Device
Part:BBa_K2082118
Designed by: Carsten Hain Group: iGEM16_Bielefeld-CeBiTec (2016-10-16)
AraC-Pbad(mod)-RBS-EPPolI-Ter
Error prone polymerase I BBa_K2082106 under control of PBAD BBa_K2082112
We expect a lower basal mutagenesis of this construct than observed in our experiment when we used BBa_K2082106 under control of a lac-promoter.
This hypothesis is not tested yet.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal XhoI site found at 1947
Illegal XhoI site found at 2445 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1347 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Illegal SapI.rc site found at 2910
[edit]
Categories
Parameters
None |