Device

Part:BBa_K2082118

Designed by: Carsten Hain   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-16)


AraC-Pbad(mod)-RBS-EPPolI-Ter

Error prone polymerase I BBa_K2082106 under control of PBAD BBa_K2082112


We expect a lower basal mutagenesis of this construct than observed in our experiment when we used BBa_K2082106 under control of a lac-promoter.
This hypothesis is not tested yet.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1947
    Illegal XhoI site found at 2445
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1347
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 2910


[edit]
Categories
Parameters
None